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CD45 Antibody Cocktail (Leukocyte marker)

Catalog Number: orb534345

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb534345
CategoryAntibodies
DescriptionCD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
ClonalityRecombinant
Species/HostRabbit
IsotypeRabbit IgG, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenRecombinant human protein was used as immunogen for this recombinant CD45 antibody cocktail.
UniProt IDP08575
Tested applicationsIHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application notesDue to variation in protocol and secondary antibody used, the recombinant CD45 antibody cocktail may need to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberPTPRC/1975R + PTPRC/1783R
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis recombinant CD45 antibody cocktail is available for research use only.
NoteFor research use only
CD45 Antibody Cocktail (Leukocyte marker)

IHC testing of recombinant CD45 antibody cocktail and FFPE human tonsil (clones PTPRC/1975R + PTPRC/1783R). Required HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

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    IHC-P,  WB

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    FACS

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    FACS

    Human

    Mouse

    Monoclonal

    CF488

    100 T