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Catalog Number | orb1936029 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB12863 |
Tested applications | FC, IF, IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, IHC-P: 1:10~50, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 81538 Da |
Target | This CD44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147~176 amino acids from the N-terminal region of human CD44. |
UniProt ID | P16070 |
NCBI | NP_001001389.1, NP_001189485.1, NP_001189484.1, NP_001189486.1, NP_001001392.1, NP_001001390.1, NP_001001391.1, NP_000601.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | CD44 antigen, CDw44, Epican, Extracellular matrix Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of HepG2 cells using CD44 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of anti-CD44 Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Formalin-fixed and paraffin-embedded human Skin tissue reacted with CD44 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CD44 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.