CD43 Antibody

• Catalog Number: orb749796
• Category: Antibodies
• Description: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Bra7G
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgM, kappa
• Immunogen: Immature pluripotent human leukemia K562 cells were used as the immunogen for the CD43 antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
• Purity: PEG precipitation
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD43 antibody is available for research use only.
• UniProt ID: P16150
• Storage: Store the CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD43 antibody should be determined by the researcher.1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at room temp2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.