CD43 Antibody

SKU: orb2641871

Description

CD43 is one of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow Cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Reactivity: Human
• Application Notes:
  Titering of the CD43 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CDLA43
• Immunogen: Recombinant full-length human protein was used as the immunogen for the CD43 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human spleen lysate (left) and human K562 cell lysate (right) with CD43 antibody (clone CDLA43). Predicted molecular weight 45-135 kDa depending on glycosylation level.

IHC testing of FFPE human spleen with CD43 antibody (clone CDLA43)

Flow cytometry testing of human K562 cells with CD43 antibody (clone CDLA43); Red = isotype control, Blue = CD43 antibody.

Immunofluorescent staining of human K562 cells with CD43 antibody (clone CDLA43, green) and NucSpot nuclear stain (red).

Quick Database Links

UniProt

P16150