CD40 (phospho Thr254) rabbit pAb

• Catalog Number: orb770942
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD40 (phospho-Thr254)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human TNFRSF5 around the phosphorylation site of Thr254. AA range:220-269
• MW: 30
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CD40 antibody, anti-TNFRSF5 antibody, anti-Tu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of COLO cells using Phospho-CD40 (T254) Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from COLO205 cells treated with Insulin 0.01U/ml 15', using TNFRSF5 (Phospho-Thr254) Antibody. The lane on the right is blocked with the phospho peptide.