CD4 mAb (GK 1.5), InVivoPure+

• Catalog Number: orb2568769
• Category: Antibodies
• Description: The monoclonal antibody GK1.5 reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by the majority of thymocytes, a subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen-presenting cells and plays an important role both in the development of T cells and in the optimal function of mature T cells. In T cells, CD4 associates with the protein tyrosine kinase p56lck via its cytoplasmic tail. The binding of GK1.5 is blocked by the antibody RM4-5, which competes with GK1.5 for the same binding site on the CD4 molecule. GK1.5 significantly increased the proportion of peripheral CD4+CD25+Foxp3+ Treg cells in CD4+ T cells in mice without altering their immunosuppressive function. The antibody profoundly blocks antigen-specific reactive mouse helper T lymphocyte lines of class II MHC.
• Target: CD4
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Rat IgG2b k
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Concentration: ≥ 0.5 mg/ mL
• Buffer/Preservatives: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Purity: ≥ 95% (CGE, reducing conditions) Endotoxin level ≤ 1 EU/mg
• Immunogen: Animals were immunized with the cloned cytotoxic T lymphocyte lines V4 and 243/2.5. L3T4 is expressed on mouse helper/inducer T cells and is analogous to the human Leu-3/T4 molecule.
• UniProt ID: P06332
• Antibody Type: Primary Antibody
• Clone Number: GK 1.5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: T-cell surface glycoprotein CD4, Cd4, T-cell diffe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.