CD3D Antibody

• Catalog Number: orb1260953
• Category: Antibodies
• Description: CD3D Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-105 of human CD3D (NP_000723.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 19kDa
• Target: CD3D
• UniProt ID: P04234
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CD3D, CD3d molecule, delta (CD3-TCR complex), CD3-
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of Jurkat cells, using CD3D antibody (orb1260953) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spleen using CD3D antibody (orb1260953) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of Jurkat cells using CD3D antibody (orb1260953) at dilution of 1:100. Blue: DAPI for nuclear staining.