CD34 Antibody

• Catalog Number: orb749892
• Category: Antibodies
• Description: This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, lambda
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the anti-CD34 antibody.
• UniProt ID: P28906
• Tested applications: FACS, IHC-P
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Application notes: Optimal dilution of the CD34 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: HPCA1/1171
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This anti-CD34 antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD34 antibody (HPCA1/1171)

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with anti-CD34 antibody (HPCA1/1171)

Flow cytometry testing of KG-1 cells using anti-CD34 antibody (red) and isotype control (green).

SDS-PAGE Analysis of Purified, BSA-Free Anti-CD34 Antibody (clone HPCA1/1171). Confirmation of Integrity and Purity of the Antibody.