CD34 Antibody

• Catalog Number: orb2635682
• Category: Antibodies
• Description: This MAb recognizes a single chain, transmembrane,heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidasebut sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human HPCA1 protein was used as the immunogen for the CD34 antibody.
• UniProt ID: P28906
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the CD34 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CD34/4939
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This CD34 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human placental tissue with CD34 antibody (clone CD34/4939) at 2 ug/ml RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with CD34 antibody (clone CD34/4939). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.