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CD31 human specific Rabbit Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:1000, Immunofluorescence 1:500 – 1:1000, Immunohistochemistry (Paraffin) 1:100 – 1:300, Immunohistochemistry (Frozen) 1:100 – 1:300, Flow Cytometry 1:50 – 1:150 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | platelet/endothelial cell adhesion molecule 1 /Synthetic peptide |
| Target | CD31 human specific |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Human peripheral blood granulocytes were stained with orb1973152, CD31 antibody (human specific).

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling CD31 with orb1973152 at 1/150.

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling CD31 with orb1973152 at 1/150.

Immunohistochemical of frozen sections. Sample: Human tissue, CD31 Antibody: 1-200, Blue: DAPI was used as the nuclear counter stain.

This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated overnight at 4°C. anti-CD31 (human specific) antibody - at 1/1000 dilution Lysates at 35 µg per lane.
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CD31 human specific Rabbit Polyclonal Antibody (orb1973152)
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