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Catalog Number | orb1973152 |
---|---|
Category | Antibodies |
Description | CD31 human specific |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human |
Immunogen | platelet/endothelial cell adhesion molecule 1 /Synthetic peptide |
Dilution range | Western Blot 1:1000 Immunofluorescence 1:500 – 1:1000 Immunohistochemistry (Paraffin) 1:100 – 1:300 Immunohistochemistry (Frozen) 1:100 – 1:300 Flow Cytometry 1:50 – 1:150 |
Conjugation | Unconjugated |
Target | CD31 human specific |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated overnight at 4°C. Anti-CD31 (human specific) antibody - at 1/1000 dilution, Lysates at 35 μg per lane.
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling CD31 with orb1973152 at 1/150.
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling CD31 with orb1973152 at 1/150.
Immunohistochemical of frozen sections. Sample: Human tissue; CD31 antibody: 1-200; Blue: DAPI was used as the nuclear counter stain.
Human peripheral blood granulocytes were stained with orb1973152, CD31 antibody (human specific).
Human | |
0.16-10 ng/mL | |
0.066 ng/mL |
98.00% | |
656 kDa (predicted); 58 kDa (reducing conditions) |