Recombinant CD31 Antibody / Rabbit Monoclonal

SKU: orb2641994

Description

CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Titering of the recombinant CD31 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: RMCD31-1
• Immunogen: A full length recombinant human protein was used as the immunogen for the recombinant CD31 antibody.
• Purification: Protein A affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human ThP1 cell lysate with recombinant CD31 antibody (clone RMCD31-1). Expected molecular weight: 83-130 kDa depending on level of glycosylation.

IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone RMCD31-1). HIER: steam sections in 1 mM EDTA, pH9, for 10-20 min.

Flow cytometry testing of PFA-fixed human Jurkat cells with recombinant CD31 antibody (clone RMCD31-1); Red = isotype control, Blue = recombinant CD31 antibody.