Recombinant CD30 Antibody / Rabbit Monoclonal

SKU: orb386231

Description

CD30 is the receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. [UniProt]

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant CD30 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: Ki-1/1505R
• Immunogen: A human partial protein was used as the immunogen for the recombinant CD30 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human Hodgkin's lymphoma stained with recombinant CD30 antibody (clone Ki-1/1505R). Required HIER: steam sections in pH9, 1 mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant CD30 antibody (clone Ki-1/1505R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P28908