CD30 Antibody

• Catalog Number: orb317424
• Category: Antibodies
• Description: Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. Its epitope is located between aa112-412. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin s disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This mAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Ber-H2
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A Co cell line established from a patient with Hodgkin s disease of T-cell lineage was used as the immunogen for the CD30 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1:50-1:100 for 30 min at RT (1)
• Purity: Unpurified high titer supernatant
• Conjugation: Other
• Formula: Bioreactor concentrate with 0.05% sodium azide
• Hazard Information: This CD30 antibody is available for research use only.
• UniProt ID: P28908
• Storage: Store the CD30 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: Bioreactor concentrate with 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD30 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human Hodgkin's lymphoma using CD30 antibody