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| Catalog Number | orb781479 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 30 Ligand(CD30L). |
| Reactivity | Rat |
| Concentration | 8000 pg/mL |
| UniProt ID | P32972 |
| Tested applications | ELISA |
| Application notes | standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD30L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD30L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD30L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD30L in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 125-8000 pg/mL |
| Sensitivity | 48 pg/mL |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | CD153 ELISA Kit, TNFSF8 ELISA Kit, CD30-L ELISA Ki Read more... |
| Note | For research use only |
