Anti-CD30 [Ki-3]

• Catalog Number: orb348919
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD30
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Ki-3
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Human CD30.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD30
• UniProt ID: P28908
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFRSF8; Tumor necrosis factor receptor superfamil
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Horn-Lohrens, O. et al. Shedding of the soluble form of CD30 from the Hodgkin-analogous cell line L540 is strongly inhibited by a new CD30-specific antibody (Ki-4) Int J Cancer, 60, 539-544 (1995)

PubMed: 7530238

Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3 Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Ki-3 (orb348919, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (orb348919). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb348919 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3, 3′-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 µg/ml.