CD276 Antibody (APC)

SKU: orb3012945

Description

CD276 Antibody (APC)

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 7-517
• Immunogen: monocyte-derived dendritic cells
• Target: CD276
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7H3, B7RP-2

Separation of human CD11c positive CD276 positive cells (red-filled) from CD11c negative CD276 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human TCR Vgamma4 (4A11.904) APC antibody (concentration in sample 0.19 µg/ml).

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD276 (7-517) APC antibody (concentration in sample 1 µg/ml).

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD11c (BU15) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD276 (7-517) APC antibody (concentration in sample 1 µg/ml).

Quick Database Links

Gene Symbol

CD276

UniProt

Q5ZPR3

NCBI Gene ID

80381