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CD273 Antibody (Biotin)
Description
Images & Validation
−| Tested Applications | FC, IHC |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | Mouse IgG2a kappa |
| Clone No. | 24F.10C12 |
| Immunogen | human CD273 cDNA |
| Target | CD273 |
| Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store at 2-8°C. Do not freeze. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Separation of CD11c positive monocytes stained using anti-human CD273 (24F.10C12) biotin antibody (concentration in sample 1.67 µg/ml, Streptavidin APC, red-filled) from CD11c positive monocytes unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) blood cells.

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) leukocytes stained using anti-human CD11c (BU15) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD273 (24F.10C12) biotin antibody (concentration in sample 1.67 µg/ml, Streptavidin APC).
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Protocol Information
CD273 Antibody (Biotin) (orb2937685)
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