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Item 1 of 4
Item 1 of 4
CD239 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1604596
| Catalog Number | orb1604596 |
|---|---|
| Category | Antibodies |
| Description | CD239 Recombinant Rabbit Monoclonal Antibody |
| Target | BCAM |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | PBS, Glycerol, rAlbumin. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD239 |
| UniProt ID | P50895 |
| MW | 64 kDa |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 1D12 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Antigen identified by monoclonal antibody F8; AU a Read more... |
| Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD239) Monoclonal Antibody, Unconjugated (orb1604596) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD239) Monoclonal Antibody, Unconjugated (orb1604596) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD239) Monoclonal Antibody, Unconjugated (orb1604596) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human TT cell lysates, Primary: Anti-CD239 (orb1604596) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kDa, Observed band size: 85 kDa.
