CD225/IFITM1 Antibody

• Catalog Number: orb1538573
• Category: Antibodies
• Description: CD225/IFITM1 Antibody
• Target: CD225 / IFITM1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 16 AA synthetic peptide near the center of human IFITM1. The immunogen is located within aa 40-90 of IFITM1.
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Application notes: Further information: IFITM1 antibody can be used for detection of IFITM1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 20 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 14 kDa Observed: 14 kDa
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFITM1, 9-27, CD225 antigen, Dispanin subfamily A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-IFITM1 antibody IHC of human uterus, myometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of IFITM1 in Jurkat cells with IFITM1 antibody at 20 ug/ml.

Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 antibody at 20 ug/ml.

Western blot analysis of IFITM1 in 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 ug/ml.