CD22 Recombinant Rabbit Monoclonal Antibody

SKU: orb1151993

Description

CD22 Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: G12H9
• Immunogen: A synthesized peptide derived from human CD22 (1-59/847aa)
• Target: CD22
• Molecular Weight: 91 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

B-cell receptor CD22; B cell receptor CD22 precursor; B lymphocyte cell adhesion molecule; BL CAM; BL-CAM; BLCAM; CD 22; CD22 antigen; CD22 molecule; CD22 protein; Leu14; Lyb8; MGC130020; Sialic acid binding immunoglobulin like lectin 2; SIGLEC 2; SIGLEC2; SIGLEC-2; FLJ22814; MGC130020; CD22_HUMAN; B-lymphocyte cell adhesion molecule; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Lysate: Daudi, Protein loading quantity: 20 µg, Exposure time: 15 s, Predicted MW: 95 kDa, Observed MW: 130 kDa.

Cell line: Daudi, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:100, Primary incubation condition: 1 hour at room temperature, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151993.

Sample: Lane 1: Human Raji cell lysates, Lane 2: Human Raji cell lysates, Primary: Anti-CD22 (orb1151993) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 91 kDa, Observed band size: 135 kDa.

Tissue: Human spleen, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151993.

Tissue: Human tonsil, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151993.

Quick Database Links

Gene Symbol

CD22

UniProt

P20273