CD209/DC-SIGN Antibody

SKU: orb1538159

Description

CD209/DC-SIGN Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC, IHC-P, IP
• Dilution Range: Flo, IHC, IHC-P (20 µg/ml), IP
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 20 ug/ml. Tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

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Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone No.: h209
• Immunogen: Human CD209
• Target: CD209 / DC-SIGN
• Purification: Affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD209, CD209 antigen, CD209 molecule, CDSIGN, CLEC4L, DC-SIGN, HIV gpl20-binding protein, DC-SIGN1

Quick Database Links

Gene Symbol

CD209 / DC-SIGN