CD2 Antibody

• Catalog Number: orb749309
• Category: Antibodies
• Description: CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific Tcell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: UMCD2
• Tested applications: FACS, Functional Studies, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Human Thymocytes/Sezary T cells were used as the immunogen for this CD2 antibody.
• Dilution range: Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 2-4ug/ml
• Purity: Protein G purified antibody
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD2 antibody is available for research use only.
• Entrez: 914
• Storage: Store the CD2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondary antibody and substrates may require the CD2 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.