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CD1E Antibody (Center)
Description
Images & Validation
−| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 43626 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
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−CD1e Antibody [orb213683]
IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
30 μl, 100 μl, 200 μl, 50 μl

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CD1E Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane).This demonstrates the CD1E antibody detected the CD1E protein (arrow).

Confocal immunofluorescent analysis of CD1E Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

CD1E Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CD1E Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD1E Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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CD1E Antibody (Center) (orb1927630)
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