CD1A Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563452
• Category: Antibodies
• Description: CD1A Recombinant Rabbit Monoclonal Antibody
• Target: CD1A
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human CD1a
• UniProt ID: P06126
• MW: 34 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: IHC-P=1:100-500, Flow-Cyt=1ug/Test, IHC-F=1:100-500, IF=1:100-500, WB=1:200-1000
• Antibody Type: Recombinant Antibody
• Clone Number: 17C20
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD 1a; CD1; CD1A Antigen; CD1A antigen, a polypept
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human Abdominal skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD1A) Monoclonal Antibody, Unconjugated (orb2563452) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD1A) Monoclonal Antibody, Unconjugated (orb2563452) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human thymus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD1A) Monoclonal Antibody, Unconjugated (orb2563452) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD1A) Monoclonal Antibody, Unconjugated (orb2563452) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.