CD19 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563456
• Category: Antibodies
• Description: CD19 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 7A6
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD19
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1000, IHC-P=1:50-200, IF=1:50-200, IHC-F=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59 kDa
• Target: CD19
• UniProt ID: P15391
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyt
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with CD19 monoclonal antibody, unconjugated (orb2563456) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD19 Monoclonal Antibody, Unconjugated (orb2563456) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.