CD19 Antibody / B-lymphocyte marker

• Catalog Number: orb385675
• Category: Antibodies
• Description: CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C19/366
• Tested applications: ELISA
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human CD19 recombinant protein was used as immunogen for this CD19 antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD19 antibody is available for research use only.
• UniProt ID: P15391
• Storage: CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.