CD177 Antibody

SKU: orb1538566

Description

CD177 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, IP, WB
• Dilution Range: ELISA, IHC, IHC-P (5 µg/ml), IP, WB
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone No.: 4C4
• Immunogen: CD177 (AAH29167, 21 aa ~ 120 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Target: CD177
• Purification: Purified from ascites by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD177, CD177 molecule, CD177 antigen, Cell surface receptor, HNA-2a, HNA2A, NB1, PRV-1, Polycythemia rubra vera 1, PRV1, NB1 glycoprotein, NB1 GP

Anti-CD177 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody, clone 4C4.

Immunoprecipitation of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead.

Detection limit for recombinant GST tagged CD177 is approximately 3 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

CD177