CD16a Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1816773
• Category: Antibodies
• Description: CD16a Recombinant Rabbit Monoclonal Antibody
• Target: FCGR3A
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Immunogen: KLH conjugated synthetic peptide derived from human IGFR3/CD16
• UniProt ID: P08637
• MW: 27 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Recombinant Antibody
• Clone Number: B12F4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD16a) Monoclonal Antibody, Unconjugated (orb1816773) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD16a) Monoclonal Antibody, Unconjugated (orb1816773) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.