CD163, Human, mAb RM3/1 Antibody

SKU: orb1108918

Description

Mouse monoclonal antibody to CD163

Images & Validation

• Tested Applications: FC, Functional Studies, IF, IHC-Fr, IP, WB
• Dilution Range: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. CD163 is sensitive to ionic detergents and to chloroform/methanol treatment.
• Reactivity: Human
• Application Notes:
  FC: Permeabilization as well as no permeabilization is used. Block cells with 1% BSA for 30';at 4°;C (Ref 5) FS: Reduction of CD69 expression by adding 2.5 µg/ml RM3/1 to 5x105 human T-lymfocytes in presence of exogenous CD163 (Ref 2). IF: Transfected CHO-cells were plated on coverslips and fixed in 4% paraformaldehyde for 2';. Cells were stained with 4 µg/ml RM3/1 for 60'. IP: 10 µg RM3/1 was covalently coupled to beads (with 20 mM dimethyl pimelimidate), blocked with 0.2 M glycine buffer for 2 hr and incubated with cell lysate for 2 hr (Ref 5). W: SDS-PAGE under non-denatured condition. Block blot with 1% skimmed milk for 1h and incubate with RM3/1 (0.4 µg/ml) in 0.1%BSA/TBS for 1h. Detection of ~130 kDa product in spleens extracted in presence of NP-40 or in glucocorticoid-treated human monocytes. (Ref 2,5)

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: Human monocytes (epitope recognized SRCR domain 9)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor