PSGL-1 Antibody / CD162

SKU: orb385968

Description

P selectin glycoprotein ligand 1 (PSGL-1), also called CD162 glycoprotein, functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the PSGL-1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: PSGL1/1601
• Immunogen: Recombinant human PSGL-1 protein was used as the immunogen for the PSGL-1 antibody.
• Purification: Protein G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human spleen with PSGL-1 antibody (clone PSGL1/1601). Recommended HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.

IHC testing of FFPE human tonsil with PSGL-1 antibody (clone PSGL1/1601). Recommended HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.

Quick Database Links

UniProt

Q14242