CD162 Antibody / P selectin glycoprotein ligand 1 / PSGL-1

• Catalog Number: orb385968
• Category: Antibodies
• Description: P selectin glycoprotein ligand 1 (PSGL-1), also called CD162 glycoprotein, functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human PSGL-1 protein was used as the immunogen for the PSGL-1 antibody.
• UniProt ID: Q14242
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Titering of the Perilipin 2 antibody may be required for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: PSGL1/1601
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This PSGL-1 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human spleen with PSGL-1 antibody (clone PSGL1/1601). Recommended HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.

IHC testing of FFPE human tonsil with PSGL-1 antibody (clone PSGL1/1601). Recommended HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.