CD146 Antibody / MCAM / MUC18

• Catalog Number: orb606461
• Category: Antibodies
• Description: CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant CD146 antibody.
• UniProt ID: P43121
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the recombinant CD146 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: rMUC18/1130
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the recombinant CD146 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This recombinant CD146 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human tonsil with CD146 antibody (clone rMUC18/1130). Required boiling tissue sections in 10mM Tris with 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant CD146 antibody (clone rMUC18/1130) as confirmation of integrity and purity.