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CD144/CDH5/VE Cadherin Antibody

SKU: orb1538157

Description

CD144/CDH5/VE Cadherin Antibody

Images & Validation

Tested ApplicationsFC, IHC, IHC-P, WB
Dilution RangeFlo, IHC, IHC-P (20 µg/ml), WB
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 20 ug/ml.Tested in Western blot using Human Umbilical Vein Endothelial Cell (HUVEC) lysates.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.16B1
ImmunogenHuman CDH5
TargetCD144 / CDH5 / VE Cadherin
PurificationAffinity purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesAqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CDH5, 7B4, 7B4 antigen, CD144, Cadherin-5, Endothelial-specific cadherin, Vascular endothelial cadherin, VE-cadherin, VEC, CD144 antigen, VE Cadherin

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol

CD144/CDH5/VE Cadherin Antibody (orb1538157)

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50 μg
$ 640.00
DispatchUsually dispatched within 2-3 weeks
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