CD14 Antibody (C-term)

• Catalog Number: orb1429920
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD14.
• Target: CD14
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: Synthetic Peptide
• UniProt ID: P08571
• MW: 40076
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB14107
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Monocyte differentiation antigen CD14, Myeloid cel
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only
• NCBI: NP_001167575.1, NP_001167576.1, NP_000582.1, NP_001035110.1
• Expiration Date: 12 months from date of receipt.

CD14 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CD14 Antibody (C-term) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the CD14 antibody detected the CD14 protein (arrow).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of CD14 (arrow) using rabbit polyclonal CD14 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2).