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Description
Research Area
Immunology & Inflammation
Images & Validation
−| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution Range | ELISA (For coating, order antibody without BSA);,Immunofluorescence (2-4ug/ml); Flow Cytometry (2-4ug/million cells); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 4-1BB/3201 |
| Immunogen | A recombinant fragment (around aa 19-188) of human CD137 protein (exact sequence is proprietary) |
| Target | TNFRSF9 |
| Source/Expression System | Bioreactor |
| Molecular Weight | 32kDa (monomer); 85kDa (dimer) |
| Purification | Protein G |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. |
| Concentration | Purified Ab conjugated to Biotin |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-4-1BB Ligand Receptor T Cell antibody, anti-Antigen 4-1BB Homolog antibody, anti-CDw137 antibody, anti-HLDA VI antibody, anti-Homolog of mouse 4 1BB antibody, anti-induced by lymphocyte activation (ILA) antibody, anti-Interleukin activated receptor homolog of mouse Ly63 antibody, anti-T-cell antigen 4-1BB / ILA homolog antibody, anti-Tumor necrosis factor receptor superfamily member 9 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)






