CD11b Antibody / MAC-1 / ITGAM

SKU: orb248418

Description

CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin alpha-M (CD11b) combines with the Integrin beta-2 (CD18) to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin alpha-M/beta-2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.

Images & Validation

• Tested Applications: FACS, Functional Studies, IF
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Functional studies (order BSA/sodium azide-free format)
• Reactivity: Human, Mouse
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD11b antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG2b, kappa
• Clone No.: M1/70
• Immunogen: B10 mouse spleen cells enriched for T lymphocytes were used as the immunogen for this CD11b antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE Analysis of Purified, BSA-Free CD11b Antibody (clone M1/70). Confirmation of Integrity and Purity of the Antibody.