CD105 Antibody (Center E395)

• Catalog Number: orb1931369
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17987
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 70578 Da
• Target: This CD105 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-409 amino acids from the Central region of human CD105.
• UniProt ID: P17813
• NCBI: NP_001265067.1, NP_000109.1, NP_001108225.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Endoglin, CD105, ENG, END
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of NCI-H292 cells using CD105 Antibody (Center E395) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CD105 antibody (Center E395) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD105 antibody (Center E395) for immunohistochemistry. Clinical relevance has not been evaluated.

Anti-CD105 Antibody (Center E395) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 95 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.