CCT007093

SKU: orb1307089

Description

CCT007093 is a potent small molecule inhibitor of the phosphatase PPM1D (WIP1), exhibiting an IC50 value of 8.4 µM. It is a valuable research tool for studying DNA damage response pathways and has been utilized in both cellular and animal models to investigate oncology and cellular stress signaling.

Research Area

Cell Biology, Metabolism Research

Key Properties

• CAS Number: 176957-55-4
• MW: 272.39
• Purity: 99.88% (May vary between batches)
• Formula: C₁₅H₁₂OS₂
• SMILES: O=C1\C(CC\C1=C/c1cccs1)=C\c1cccs1
• Target: Autophagy,Phosphatase,Apoptosis
• Solubility: DMSO:Slightly soluble

Bioactivity

• Target IC50:
  PPM1D:8.4 μM
• In Vivo:
  CCT007093, when used in combination with Paclitaxel, synergistically inhibits four Paclitaxel-resistant triple-negative breast cancer cell lines. CCT007093 selectively exhibits significant inhibition on human tumor cell lines over-expressing PPM1D (MCF-7, KPL-1, and MCF-3B). By activating p38 kinase activity, CCT007093 induces cell death. It selectively promotes apoptosis in breast cancer cells and ectopically expressed Wip1 in keratinocytes, while reducing UV-mediated apoptotic responses in Wip1-deficient naked cells and a skin keratinocyte model.
• Cell Research:
  Cells are transfected with a pSUPER plasmid and an additional plasmid expressing the blasticidin resistance gene (pEFBsd) in a molar ratio of 10:1. Cells are plated in 10 cm plates 24 h after transfection. Blasticidin selection (5 μg/ml) is initiated 48 h post-transfection and replenished every 3 days. Colonies are fixed in methanol and stained with crystal violet after 14 days. Colonies are quantified on a Colcount and the surviving fraction (SF) determined.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCT 007093, CCT007093, CCT-007093, Autophagy, Apoptosis, Inhibitor, inhibit, PPM1D, Phosphatase

Compound Identifiers

PubChem CID

2314623