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Catalog Number | orb55497 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against CCR2 conjugated to FITC. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC |
Reactivity | Human, Rat |
Immunogen | Synthetic peptide of Human CCR2 |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000); IHC (1:50) |
Conjugation | FITC |
MW | 42 kDa |
Target | CCR2 |
Entrez | 729230 |
UniProt ID | P41597 |
NCBI | NP_001116513.2 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS |
Alternative names | CKR2 antibody, C-C chemokine receptor type 2 antib Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SPC-704 was sufficient for detection of CCR2 in 15 µg of rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry analysis using Rabbit Anti-CCR2 Polyclonal Antibody. Tissue: Tonsil. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa.
WB | |
Bovine, Canine, Equine, Human | |
Rabbit | |
Polyclonal | |
FITC |
WB | |
Human, Porcine, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
FITC |