CCL8/MCP2 Antibody

• Catalog Number: orb1539111
• Category: Antibodies
• Description: CCL8/MCP2 Antibody
• Target: CCL8 / MCP2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Lyophilized from PBS. Lyophilized from PBS
• Purification: Protein A purified
• Immunogen: E.coli derived recombinant Human MCP-2 cprresponding to amino acids 24-99 of P80075.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: ELISA (2 - 4 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.25 - 0.5 µg/ml)
• Application notes: Resuspension: Reconstitute with sterile water to a concentration of 1 mg/ml.. Further information: Immunohistochemistry: LS-B8198 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human MCP-2 when used with biotinylated antigen affinity purified anti-human MCP-2 (LS-C104731) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CCL8, C-C motif chemokine 8, HC14, MCP2, Monocyte
• Note: For research use only
• Expiration Date: 12 months from date of receipt.