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| Catalog Number | orb1537808 |
|---|---|
| Category | Antibodies |
| Description | CCL5/RANTES Antibody |
| Target | CCL5 / RANTES |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS. PBS |
| Purification | Protein A purified |
| Immunogen | RANTES |
| Tested applications | ELISA, IHC, IHC-P |
| Dilution range | ELISA (2 - 4 µg/ml), IHC, IHC-P (5 µg/ml) |
| Application notes | Further information: Immunohistochemistry: LS-B2472 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2472 was determined to be 5 ug/ml. Neutralization: 5-7 ug/ml to yield maximum inhibition (ND50) of hRANTES at 100 ng/ml). |
| Clone Number | 8.PP.11 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CCL5, Chemokine (C-C motif) ligand 5, D17S136E, Eo Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
IF, IHC-Fr, IHC-P | |
Bovine, Equine, Human, Mouse, Porcine | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC | |
Human, Monkey, Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC, WB | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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