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Catalog Number | orb1538966 |
---|---|
Category | Antibodies |
Description | CCL20/MIP-3-Alpha Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ELISA, IHC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1,k |
Immunogen | E.coli derived recombinant Human MIP-3alpha. |
Dilution range | ELISA (2 - 4 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml) |
Conjugation | Unconjugated |
Target | CCL20 / MIP-3-Alpha |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Lyophilized from PBS. Lyophilized from PBS |
Alternative names | CCL20, Beta chemokine exodus-1, Beta-chemokine exo Read more... |
Note | For research use only |
Application notes | Resuspension: Centrifuge the vial before opening. Reconstitute in sterile water to 0.1 mg/ml.. Further information: Immunohistochemistry: LS-B7409 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3alpha when used with biotinylated antigen affinity purified anti-Human MIP-3alpha (LS-C104612) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 0.25-0.50 ng/lane, under non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |