CCK8 Rabbit Polyclonal Antibody Discontinued

SKU: orb156288

Description

CCK8 Rabbit Polyclonal Antibody

Research Area

Hormone Research, Obesity

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Gallus, Human, Mouse, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CCK8 (96-103/115aa)
• Target: CCK
• Molecular Weight: 12.6 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCK 8; CCK; CCK8; CCK-8, Cholecystokinin 8; Cholecystokinin; Cholecystokinin preproprotein; Cholecystokinin8; Cholecystokinins precursor; MGC117187; Procholecystokinin precursor.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CCK8 Polyclonal Antibody, Unconjugated (orb156288) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CCK

UniProt

P06307

Qi He 1, Lin Jiang 2, Feng-Lei Chao 3, Chun-Ni Zhou 3, Lei Zhang 3, Yu-Ning Zhou 3, Yi-Ying Wang 3, Yu-Hui Deng 3, Jing Tang 3, Yanmin Luo 3, Xin Liang 3, Jing Li 3, Yi Zhang 4, Yong Tang The role of LINGO-1 in regulating CB1R/TrkB signalling and GABAergic interneurons in Alzheimer's disease pathogenesis Exp Neurol, (2025)

PubMed: 40441519

Applications

IF

Reactivity

Mouse