CCG-1423

SKU: orb1306739

Description

CCG-1423 is a potent small molecule inhibitor that selectively targets the RhoA signaling pathway, thereby inhibiting Serum Response Factor (SRF)-mediated gene transcription. It is a valuable research tool for studying Rho GTPase pathways in cellular models of fibrosis, cancer metastasis, and cardiovascular function, with demonstrated utility in both in vitro and in vivo experimental systems.

Research Area

Cell Biology, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 285986-88-1
• MW: 454.75
• Purity: 99.98%
• Formula: C₁₈H₁₃ClF₆N₂O₃
• SMILES: CC(ONC(=O)c1cc(cc(c1)C(F)(F)F)C(F)(F)F)C(=O)Nc1ccc(Cl)cc1
• Target: LRRK2,Ras,Apoptosis,Rho
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (7.26 mM);DMSO:245 mg/mL (538.76 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:4 mg/mL (8.8 mM)

Bioactivity

• Target IC50:
  LRRK2 (G2019S):1.85 nM|LRRK2 (WT):1.28 nM
• In Vivo:
  In H9c2 cells, CCG-1423 inhibits MRTF nuclear localization and completely blocks the activity of STARS proximal reporter genes. It also suppresses TGF-β-induced fibrogenesis in human colon myofibroblasts. Under the influence of CCG-1423 and LY294002, mouse embryonic stem cells differentiate into the mesendoderm. In melanoma cells (A375M2 and SK-Mel-147) overexpressing RhoC, CCG-1423 selectively inhibits cell proliferation.
• Cell Research:
  Cells in normal culture medium are plated (2,000 per well) in a 96-well plate coated with laminin. After attachment, the medium is replaced with serum-free medium (0% FBS) with 30 μmol/L LPA with or without 300 nM CCG-1423. Fresh LPA with or without CCG-1423 is added at day 5 to ensure that LPA and compound are present throughout the experiment. On day 8, WST-1 reagent is added to the wells for 1 h and absorbance at 450 nm is read using a Victor plate reader.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

bioisostere, Apoptosis, conformational restriction, CCG 1423, CCG1423, CCG-1423, glucose tolerance, G2019S LRRK2, Inhibitor, LRRK2 (WT), metastasis, PC-3, mice, inhibit, Ras, prostate,serum, RhoA

Compound Identifiers

PubChem CID

2726015