CCG-1423

SKU: orb1225430

Description

CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.

Key Properties

• CAS Number: 285986-88-1
• MW: 454.75
• Purity: >98% (HPLC)
• Formula: C₁₈H₁₃ClF₆N₂O₃
• SMILES: O=C(NOC(C)C(NC1=CC=C(Cl)C=C1)=O)C2=CC(C(F)(F)F)=CC(C(F)(F)F)=C2
• Target: Rho
• Solubility: Ethanol: 4 mg/mL warmed (8.79 mM); DMSO: 90 mg/mL (197.91 mM)

Bioactivity

• In Vivo:
  Animal model: 16-week-old mice with HFD-induced obesity. Dosage: 0.15 mg/kg. Administration: Intraperitoneal injection; 0.15 mg/kg once per day; for two weeks. Result: Improved glucose tolerance and reduced insulin levels at 30 minutes after glucose injection.
• In Vitro:
  Cell Invasion Assay Cell line: PC-3 cell line. Concentration: 10 μM. Incubation time: 24 hours. Result: Inhibited 71% invasion by cultured PC-3 cells into a Matrigel matrix. Western blot analysis. Cell line: L6 myotubes. Concentration: 1 μM. Incubation time: 48 hours. Result: Increased insulin-stimulated Akt phosphorylation, blocked ERK phosphorylation, and increased IRS-1 tyrosine phosphorylation and its association with the p85 regulatory subunit of PI3K. Cell Proliferation Assay Cell line: PC-3 cell line. Concentration: 0.3 μM Incubation time: 8 days. Result: Inhibited growth of PC-3 prostate cancer cells with an IC50 value of 1 μM with 30 μM LPA adding. Apoptosis Analysis Cell line: RhoC-overexpressing A375M2 and low RhoC-expressing A375 melanoma cell lines. Concentration: 3 μM. Incubation time: 25 hours. Result: Stimulated apoptosis of A375M2 cell line compared with the parental cell line.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCG1423 | CCG-1423 | CCG 1423

Quick Database Links

Gene Symbol

Rho