CCDC69 Antibody

SKU: orb1240024

Description

CCDC69 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  WB: 1-2 μg/mL; IF: 5-20 μg/mL; IHC: 1-5 μg/mL. Antibody validated: Western Blot in human, mouse and ratsamples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: Anti-CCDC69 antibody was raised against an 18 amino acid synthetic peptide from near the center of human CCDC69. The immunogen is located within amino acids 150 - 200 of CCDC69.
• Target: CCDC69
• Molecular Weight: Predicted: 35kDObserved: 35 kD
• Purification: CCDC69 Antibody is affinity chromatography purified via peptide column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: CCDC69 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

CCDC69 Antibody: Coiled-coil domain-containing protein 69

WB Validation in Human Cell Lines, Loading: 15 µg of lysate. Antibodies: CCDC69 orb1240024, 1 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Pancreas Tissue Lysate, Loading: 15 µg of lysates per lane. Antibodies: CCDC69 orb1240024, 1 µg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Pancreas Lysate, Loading: 15 µg of lysates per lane. Antibodies: CCDC69 orb1240024, 1 µg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry of CCDC69 in human lung tissue with CCDC69 antibody at 5 µg/mL.

Immunofluorescence of CCDC69 in human lung tissue with CCDC69 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

CCDC69

UniProt

A6NI79

RefSeq (Protein):NP_056436