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Catalog Number | orb1240024 |
---|---|
Category | Antibodies |
Description | CCDC69 Antibody |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Predicted Reactivity | Bovine |
Reactivity | Human, Mouse, Rat |
Isotype | IgY |
Immunogen | Anti-CCDC69 antibody was raised against an 18 amino acid synthetic peptide from near the center of human CCDC69. The immunogen is located within amino acids 150 - 200 of CCDC69. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 35kDObserved: 35 kD |
Target | CCDC69 |
UniProt ID | A6NI79 |
NCBI | NP_056436 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | CCDC69 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | CCDC69 Antibody: Coiled-coil domain-containing pro Read more... |
Note | For research use only |
Application notes | WB: 1-2 μg/mL; IF: 5-20 μg/mL; IHC: 1-5 μg/mL. Antibody validated: Western Blot in human, mouse and ratsamples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
WB Validation in Human Cell Lines, Loading: 15 µg of lysate. Antibodies: CCDC69 orb1240024, 1 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Pancreas Tissue Lysate, Loading: 15 µg of lysates per lane. Antibodies: CCDC69 orb1240024, 1 µg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Pancreas Lysate, Loading: 15 µg of lysates per lane. Antibodies: CCDC69 orb1240024, 1 µg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of CCDC69 in human lung tissue with CCDC69 antibody at 5 µg/mL.
Immunofluorescence of CCDC69 in human lung tissue with CCDC69 antibody at 20 µg/mL.
WB | |
Bovine, Canine, Equine, Guinea pig, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Porcine, Rat | |
Rabbit | |
Polyclonal | |
HRP |
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Porcine, Rat | |
Rabbit | |
Polyclonal | |
FITC |