CCDC59 Antibody

SKU: orb1259969

Description

CCDC59 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human CCDC59 (NP_054886.2).
• Target: CCDC59
• Molecular Weight: Observed: 29kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

BR22, HSPC128, TAP26, thyroid transcription factor 1-associated protein 26, TTF-1-associated protein 26, TTF-1-associated protein BR2, coiled-coil domain-containing protein 59

Western blot analysis of extracts of various cell lines, using CCDC59 antibody (orb1259969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat liver using CCDC59 antibody (orb1259969) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using CCDC59 antibody (orb1259969) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using CCDC59 antibody (orb1259969) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using CCDC59 antibody (orb1259969) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using CCDC59 antibody (orb1259969) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using CCDC59 antibody (orb1259969) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using CCDC59 antibody (orb1259969) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH-3T3 cells using CCDC59 antibody (orb1259969) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using ESRRA antibody (orb1260061) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

CCDC59

UniProt

Q9P031