CCDC3 Antibody

• Catalog Number: orb1248408
• Category: Antibodies
• Description: CCDC3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-PHGVNFQDAIFPDTQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected HEK293 transiently expressing Human CCDC3 (DYKDDDDK tagged), a band of approx. 36kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-DYKDDDDK antibody. Recommended concentration: 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CCDC3
• UniProt ID: Q9BQI4
• NCBI: NP_113643.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CCDC3, coiled-coil domain containing 3, coiled-coi
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected HEK293 transiently expressing Human CCDC3 (DYKDDDDK tagged), a band of approx. 36kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-DYKDDDDK antibody. Recommended concentration: 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing Human CCDC3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with orb1248408 (0.5 ug/ml) in the right panel (empty vector transfection in first lanes).

orb1248408 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.