CBR1 Antibody

• Catalog Number: orb1247699
• Category: Antibodies
• Description: CBR1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HGQFVSEKRVEQW
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 30-35kDa band observed in lysates of Glioblastoma cell line U251 and in Human Kidney lysates (calculated MW of 30.4kDa according to NP_001748.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration: 1-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CBR1
• UniProt ID: P16152
• NCBI: NP_001748.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CBR1, carbonyl reductase 1, CBR, carbonyl reductas
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 30-35kDa band observed in lysates of Glioblastoma cell line U251 and in Human Kidney lysates (calculated MW of 30.4kDa according to NP_001748.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration: 1-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247699 (0.1 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247699 (3 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247699 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1247699 (0.1 ug/ml) staining of U251 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.