CBFB Antibody (Center)

• Catalog Number: orb1929725
• Category: Antibodies
• Description: CBFB Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q13951
• MW: 21508 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Note: For research use only

CBFB Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CBFB Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

CBFB Antibody (Center) western blot analysis in K562 cell line and mouse lung tissue lysates (35 ug/lane). This demonstrates the CBFB antibody detected the CBFB protein (arrow).

All lanes: Anti-CBFB Antibody (Center) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.