CBFA1/RUNX2 Antibody

SKU: orb1538295

Description

CBFA1/RUNX2 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Reactivity: Human, Rat
• Application Notes:
  Further information: Western Blot (Cell lysate) - positive control PC-12. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone No.: 3F5
• Immunogen: RUNX2 (NP_004339, 251 aa ~ 351 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Target: CBFA1 / RUNX2
• Purification: Purified from ascites by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4. PBS, pH 7.4
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

RUNX2, AML3, CCD, CCD1, OSF2, PEBP2A1, PEBP2A2, PEA2-alpha A, PEBP2-alpha A, OSF-2, PEBP2A, PEBP2aA1, CBF-alpha-1, CBFA1, Oncogene AML-3, PEA2aA, PEBP2aA

Immunofluorescence of monoclonal antibody to RUNX2 on HeLa cell (antibody concentration 10 ug/ml).

RUNX2 monoclonal antibody, clone 3F5 Western blot of RUNX2 expression in PC-12.

Detection limit for recombinant GST tagged RUNX2 is approximately 0.1 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

CBFA1 / RUNX2